template dna molecule Search Results


dna template molecules  (GenScript corporation)
90
GenScript corporation dna template molecules
Bait-reporter <t>DNA</t> templates for T7-transcription were created by subcloning designed sxRNA bait and bait-reporter sequences into a pUC57 based plasmid using demarcated restriction <t>sites</t> <t>(GenScript).</t> The region for the insertion corresponds to the 3′UTR of the transcribed, artificial mRNA. Figure 4 depicts vector bait reporter vector 2 (BRV-2), used for all bait reporters other than BR-1a and BR-1b, that both use bait reporter vector 1. BRV-1 has 64 bases 3′ of the luciferase stop codon and 5′ of the switch insert, whereas BRV-2 has only 12 bases in this position and their composition differs from BRV-1 (see ). Restriction site denoted with asterisk is used to linearize plasmid to allow for polymerase “runoff” based termination of transcription. The prototypic architecture of a final, capped, T7-transcribed sxRNA (mRNA) is represented at the bottom.
Dna Template Molecules, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/template+dna+molecule/dna+template+molecules/pmc05368982-136-0-12
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dna template molecules - by Bioz Stars, 2026-09
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1d sequencing of template and complement strands of a single dna molecule  (Metrichor Ltd)
90
Metrichor Ltd 1d sequencing of template and complement strands of a single dna molecule
Bait-reporter <t>DNA</t> templates for T7-transcription were created by subcloning designed sxRNA bait and bait-reporter sequences into a pUC57 based plasmid using demarcated restriction <t>sites</t> <t>(GenScript).</t> The region for the insertion corresponds to the 3′UTR of the transcribed, artificial mRNA. Figure 4 depicts vector bait reporter vector 2 (BRV-2), used for all bait reporters other than BR-1a and BR-1b, that both use bait reporter vector 1. BRV-1 has 64 bases 3′ of the luciferase stop codon and 5′ of the switch insert, whereas BRV-2 has only 12 bases in this position and their composition differs from BRV-1 (see ). Restriction site denoted with asterisk is used to linearize plasmid to allow for polymerase “runoff” based termination of transcription. The prototypic architecture of a final, capped, T7-transcribed sxRNA (mRNA) is represented at the bottom.
1d Sequencing Of Template And Complement Strands Of A Single Dna Molecule, supplied by Metrichor Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/template+dna+molecule/1d+sequencing+of+template+and+complement+strands+of+a+single+dna+molecule/bio_rxiv__046086-7-7-0
Average 90 stars, based on 1 article reviews
1d sequencing of template and complement strands of a single dna molecule - by Bioz Stars, 2026-09
90/100 stars
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Bait-reporter DNA templates for T7-transcription were created by subcloning designed sxRNA bait and bait-reporter sequences into a pUC57 based plasmid using demarcated restriction sites (GenScript). The region for the insertion corresponds to the 3′UTR of the transcribed, artificial mRNA. Figure 4 depicts vector bait reporter vector 2 (BRV-2), used for all bait reporters other than BR-1a and BR-1b, that both use bait reporter vector 1. BRV-1 has 64 bases 3′ of the luciferase stop codon and 5′ of the switch insert, whereas BRV-2 has only 12 bases in this position and their composition differs from BRV-1 (see ). Restriction site denoted with asterisk is used to linearize plasmid to allow for polymerase “runoff” based termination of transcription. The prototypic architecture of a final, capped, T7-transcribed sxRNA (mRNA) is represented at the bottom.

Journal: Scientific Reports

Article Title: Engineering Structurally Interacting RNA (sxRNA)

doi: 10.1038/srep45393

Figure Lengend Snippet: Bait-reporter DNA templates for T7-transcription were created by subcloning designed sxRNA bait and bait-reporter sequences into a pUC57 based plasmid using demarcated restriction sites (GenScript). The region for the insertion corresponds to the 3′UTR of the transcribed, artificial mRNA. Figure 4 depicts vector bait reporter vector 2 (BRV-2), used for all bait reporters other than BR-1a and BR-1b, that both use bait reporter vector 1. BRV-1 has 64 bases 3′ of the luciferase stop codon and 5′ of the switch insert, whereas BRV-2 has only 12 bases in this position and their composition differs from BRV-1 (see ). Restriction site denoted with asterisk is used to linearize plasmid to allow for polymerase “runoff” based termination of transcription. The prototypic architecture of a final, capped, T7-transcribed sxRNA (mRNA) is represented at the bottom.

Article Snippet: DNA template molecules (see for sequence details) were designed and produced commercially (GenScript, USA). sxRNA vector constructs were linearized using BtgI (New England Biolabs Inc.) following the standard protocol.

Techniques: Subcloning, Plasmid Preparation, Luciferase